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DNA萃取─純化─放大整合晶片
A DNA-extraction and Polymerase-chain-reaction Microchip Using Magnetic Beads and Thermo-pneumatic Valves


賈婷婷、楊欣穎、鄭茗元、楊燿州 教授


      We develop a miniaturized system for DNA extraction and polymerase chain reaction (PCR).  DNA is extracted from whole blood sample by using silica coated magnetic beads.  Electromagnetic actuation is employed to manipulate the magnetic beads for transporting DNA molecules in fluidic channels.  Thermo-pneumatic valves with low temperature elevation are integrated into the microfluidic chip for isolating/releasing fluidic samples and reagents.  The size of the system is 72mm×54mm×11mm.  The device fully operates with a 12V DC voltage and automatically performs DNA extraction and amplification processes after samples and reagents are loaded.


 


研究著作:

Bonnie T. Chia, X.-Y. Yang, M.-Y. Cheng, and Y.-J. Yang, “A DNA-extraction and Polymerase-chain-reaction Microchip Using Magnetic Beads and Thermo-pneumatic Valves,” IEEE MEMS 2010, Hong Kong, Jan. 24-28, 2010 .
 

   

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