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整合微型電磁致動器之聚合酶連鎖反應晶片
A Self-contained Miniaturized PCR System
賈婷婷、楊勝安、鄭茗元、林俊良、林啟萬 教授、楊燿州 教授
The development of a portable polymerase chain reaction (PCR) device is presented. The fully integrated self-contained system consists of four major parts: a disposable chamber chip with micro-channels and pumping membranes, a heater chip with micro-heaters and temperature sensors, a linear array of electromagnetic actuators, and a control/sensing circuit. The system can be fully operated with a 5V DC voltage, and does not require any external air compressor or bulky power supply. The size of the whole system is 67mm × 66mm × 25mm, and is smaller than a PDA cell-phone. The miniaturized PCR system not only has the advantage of smaller size, less consumption of DNA solution, but also can effectively reduce the PCR process time into one-third of the time required by typical commercial PCR system.
研究著作:
B T. Chia, S.-A. Yang, M.-Y. Cheng, C.-L. Lin, C.-W. Lin and Y.-J. Yang, “A Self-contained Miniaturized PCR System,” IEEE MEMS 2009, Sorrento, Italy, Jan. 25-29, 2009.
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電磁驅動式聚合酶連鎖反應微晶片系統
A Self-contained Miniaturized PCR System Using Electromagnetic Actuators
賈婷婷 楊勝安 鄭茗元 林俊良
發表於 IEEE MEMS Conference 2009, Sorrento, Italy.
聚合酶連鎖反應(PCR)為生醫領域中體外擴增基因片段的重要技術，常見應用於DNA定序、醫療診斷、疾病檢測等。
透過微機電技術(micro-electro-mechanical system, MEMS)製作的微流道晶片(microfluidic chip)與微加熱器晶片(micro heater chip)，再加上控制電路(control/sensing module)與流體驅動元件(electromagnetic actuator)所組成之PCR微系統體積比PDA手機還小，僅需接上電源即可使用，可減少反應時間、減少DNA使用量，成功取代了大型實驗操作機台。
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