A DNA-extraction and Polymerase-chain-reaction Microchip using Magnetic Beads and Thermo-pneumatic Valves

Ting-Ting Chia, Xing-Ying Yang, Ming-Yuan Cheng, and Yao-Joe Yang

      We develop a miniaturized system for DNA extraction and polymerase chain reaction (PCR).  DNA is extracted from whole blood sample by using silica coated magnetic beads.  Electromagnetic actuation is employed to manipulate the magnetic beads for transporting DNA molecules in fluidic channels.  Thermo-pneumatic valves with low temperature elevation are integrated into the microfluidic chip for isolating/releasing fluidic samples and reagents.  The size of the system is 72mm×54mm×11mm.  The device fully operates with a 12V DC voltage and automatically performs DNA extraction and amplification processes after samples and reagents are loaded.

 

Publication:
Bonnie T. Chia, X.-Y. Yang, M.-Y. Cheng, and Y.-J. Yang, “A DNA-extraction and Polymerase-chain-reaction Microchip Using Magnetic Beads and Thermo-pneumatic Valves,” IEEE MEMS 2010, Hong Kong, Jan. 24-28, 2010 .